Clinical Study Results: THREE is Backed by Science


At THREE, we're backed by science and what that means is that actual scientists, scholars, and doctors study everything from the plants that make our products, to the rate at which our body absorbs the nutrients within them. We believe that true wellness begins at the cellular level. It's not just about what you consume, but how effectively your body absorbs and utilizes nutrients. That's why we have invested in innovative technologies that enhance absorption and deliver nutrients directly to the cells where they are needed most. Our cutting-edge formulations in all six of our wellness products are designed to ensure optimal bioavailability, allowing your body to fully absorb and benefit from the nutrients.

We wanted to put our products to the test to prove the science behind our formulations. To do this, we put the entire THREE product line through a clinical study to determine the cellular absorption and bioavailability of our products. Our products talk the talk, but do they walk the walk? The results of this study helped us determine at what percentage the nutrients included in the THREE product line being absorbed by the body and well, it’s safe to say we’re pretty excited about the results.

Consider yourself warned, there’s a decent amount of science specific jargon as we publish the study below, we love to nerd out about that stuff over here! If you want to do a TL:DR (too long, didn’t read), you can click the button below to download a summary plus the full study to share with your family and friends and anyone who’s ready to live and feel their best THREE life!

The Purpose of the Study

If a nutritional supplement is to enhance human health, the bioactive ingredients in the product need to cross the cellular membrane at a high concentration to realize this objective.

The problem is this: the bioactive molecules in nutritional supplements are predominantly organic molecules from plants and are hydrophobic (i.e. they are afraid of and are not soluble in water). Because the body is made up of 60% water (the blood is 90% water), these bioactive molecules are poorly absorbed into the cell and have low bioavailability.

General absorption and cellular bioavailability of organic molecules is ~10%.

THREE has developed and employs many different Cellular Absorption Technologies in its products to increase cellular absorption and bioavailability.

The purpose of this clinical study was to determine the cellular absorption and bioavailability of THREE’s product line.

three scientific study shows high bioavailability

Methods Behind the Study

Cellular absorption and bioavailability was determined by use of a Caco-2 permeability assay using 0.4 μM pore size in a 12-well plate format. Filter was pre-incubated with media for 30 minutes before seeding the cells. 100 μL apical, 600 μL basal. 3X10^5 cells were plated per insert. Media volume was set to 0.5 mL apical and 1.6 mL basal. Media was replaced every 2-3 days until tight junction formation. Products were tested at the concentrations, durations, and then quantified at the absorption ranges shown in Table 1 below.

three supplements sitting on a table

Results of the Study

Predicted cellular absorption and bioavailability percentages of THREE’s products are shown in Figure 1 below. Actual numbers are as follows: Éternel = 95%, Purifí = 88%, Collagène = 73%, Vitalité = 44%, Imúne = 44%, Revíve = 30%, and curcumin (Organic Control) = 10.

clinical study of three supplements show high bioavailability

The correct way to analyze the data in Figure 1 is to compare the percent absorption of each product to the organic control—and not between products.

When looked in this way, Revíve, with a cellular absorption percentage of 30, is 3X more bioavailable than curcumin (organic control), which represents a standard organic molecule and served as the control in the experiment. Éternel is 9.5X more bioavailable than curcumin. The significant increases in cellular absorption and bioavailability in THREE’s products versus a standard molecule like curcumin (organic control) is due to the Cellular Absorption Technologies used in THREE’s products that help to shuttle the ingredients in the formulas across the cellular membrane and into the cell at a much higher concentration.

THREE Products are up to 9.5X More Absorbable

A Caco-2 in-vitro cellular absorption assay was used to calculate percent absorption and bioavailability of THREE’s products.

The data found that THREE’s products, due to the Cellular Absorption Technologies used, are between 3 and 9.5 times more absorbable than a standard organic molecule using no delivery technology. Increased cellular absorption and bioavailability should equate to increased product efficacy.

Summary of the Study

three products are 3 to 9.5 times more absorbable than standard molecules

The purpose of this clinical study was to determine the cellular absorption and bioavailability of THREE’s product line, meaning, at what percentage are the nutrients included in THREE’s product line being absorbed by the body?

The results of this study indicate that due to the Cellular Absorption Technologies used in formulation and manufacturing, THREE’s products are between 3 and 9.5 times more absorbable than the standard organic control molecule used as a baseline in the study.